Exploration on the Mechanism of PPARα on Regulating the SOD2 Expression

doi:10. 3969 / j. issn. 2096-2266. 2018. 02. 007

Abstract

Abstract: Objective: To investigate the mechanism of PPARα on regulating the SOD2 expression. Methods: The BMEC and the
mice C57BL/6 were treated with fenofibrate. PPARα and SOD2 expression in BMEC and the brain microvessel of the mice C57BL/6
which treated with fenofibrate were tested by the real-time PCR. Luciferase reporter plasmid including the promoter of SOD2 was
named as pGL4 mSOD2 and the promoter of SOD2 which PPRE domain was mutated was named as pGL4 mu mSOD2. The activity of
Luciferase in BMEC which was co-transfected with Luciferase reporter system including pGL4 mSOD2, or pGL4 mu mSOD2 with
pGL4 was tested. Results: The expression of PPARα and SOD2 were enhanced in the BMEC or mice C57BL/6 treated with fenofibrate.
The activity of Luciferase in BMEC was promoted in the Luciferase reporter system including pGL4 mSOD2 when the fenofibrate was
used; however, there was no change of the activity of Luciferase in the Luciferase reporter system including pGL4 mu mSOD2 even
though the BMEC was treated with fenofibrate. Conclusion: Fenofibrate regulates the SOD2 expression by activating PPARα,
activated PPARα combine with pGL4 mSOD2 PPRE.

Key words: fenofibrate, peroxisome proliferator-activated receptor, superoxide dismutase

Yang Xitong, Qin Yan, Du Xiaoshan, Xu Hongyang, Wang Guangming. Exploration on the Mechanism of PPARα on Regulating the SOD2 Expression[J]. Journal of Dali University, DOI: 10. 3969 / j. issn. 2096-2266. 2018. 02. 007.

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